Mannitol Yeast Extract Medium (LMG 135)

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Yeast Extract Medium
(LMG 135)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Mannitol......................................................................................10.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
NaCl..............................................................................................0.1g
CaCl 2 ·2H 2 O solution..................................................................1.0mL
FeCl 3 ·6H 2 O solution..................................................................1.0mL
CaCl 2 ·2H 2 O Solution:
Composition per 10.0mL:
CaCl 2 ·2H 2 O..............................................................................5.28mg
Preparation of CaCl 2 ·2H 2 O Solution: Add CaCl 2 ·2H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
FeCl 3 ·6H 2 O Solution:
Composition per 10.0mL:
FeCl 3 ·6H 2 O..............................................................................0.66mg
Preparation of FeCl 3 ·6H 2 O Solution: Add FeCl 3 ·6H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Rhizobium species and
Bradyrhizobium species.