Marinithermus hydrothermalis Medium (DSMZ Medium 973)

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marinithermus hydrothermalis Medium
(DSMZ Medium 973)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................30.0g
MgCl 2 ·6H 2 O ...............................................................................4.18g
MgSO 4 ·7H 2 O................................................................................3.4g
Yeast extract..................................................................................1.0g
Tryptone........................................................................................1.0g
KCl..............................................................................................0.33g
NH 4 Cl .........................................................................................0.25g
K 2 HPO 4 .......................................................................................0.14g
CaCl 2 ·2H 2 O ................................................................................0.14g
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O.............................................................10.0mg
NiCl 2 ·6H 2 O................................................................................0.5mg
Na2Se 3 ·5H 2 O.............................................................................0.5mg
Trace elements solution...........................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O..........................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Marinithermus hydrothermalis.