Marinitoga Medium (DSMZ Medium 904)

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marinitoga Medium
(DSMZ Medium 904)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1045.0mL:

Sea salts ......................................................................................30.0g
PIPES............................................................................................6.0g
Yeast extract..................................................................................1.0g
Tryptone........................................................................................1.0g
Resazurin ...................................................................................0.5mg
Glucose solution ......................................................................25.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
L -Cysteine solution..................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 25.0mL:
Glucose.........................................................................................2.5g
Preparation of Glucose Solution:  Add sucrose to distilled/deion-
ized water and bring volume to 25.0mL. Mix thoroughly. Sparge with
100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
L -Cysteine Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H 2 O .....................................................................0.5g
Preparation of  L -Cysteine Solution:  Add  L -cysteine·HCl·H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.5g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Prepare and dispense medium under 100%
N 2 . Add components, except glucose solution,  L -cysteine-HCl·H 2 O so-
lution, and Na 2 S·9H 2 O solution, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into anaer-
obe tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically and anaerobically add per liter, 50.0mL glucose solution,
10.0mL  L -cysteine-HCl·H 2 O solution, and 10.0mL Na 2 S·9H 2 O. Mix
thoroughly. The final pH should be 7.0.
Use: For the cultivation of Marinitoga camini and Caloranaerobacter
azorensis.