MBM Medium (Modified) (DSMZ Medium 1020)

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

MBM Medium (Modified)
(DSMZ Medium 1020)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaNO 3 ...........................................................................................0.2g
KH 2 PO 4 ........................................................................................0.2g
NH 4 Cl ..........................................................................................0.2g
MgCl 2 ·6H 2 O..................................................................................0.4g
KCl ...............................................................................................0.2g
CaCl 2 ·2H 2 O...................................................................................0.1g
Resazurin ..................................................................................1.0mg
Thiosulfate solution .................................................................10.0mL
Trace elements solution SL-4 ...................................................2.0mL
pH 7.0 ± 0.2 at 25°C
Thiosulfate Solution:
Composition per 10.0mL:
Na 2 S 2 O 3 ........................................................................................2.5g
Preparation of Thiosulfate Solution:  Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N 2 . Filter sterilize.
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O................................................................................. 0.2g
Trace elements solution SL-6 ................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sparge with 100% N 2 . Filter sterilize.
Preparation of Medium: Add components, except thiosfulfate so-
lution and trace elements SL-4 solution, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Sparge solution with
80% N 2 and 20% CO 2 gas mixture to make it anoxic. Distribute into
culture vessels (e.g., 20mL in 120mL serum bottles) under a gas atmo-
sphere of 80% N 2 and 20% CO 2 . Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to room temperature. Prior to inoculation, add the
trace elements solution and thiosulfate solution to the medium. Adjust
pH to 7.0. After inoculation, pressurize culture vessels to 0.5 bar over-
pressure with 100% H 2 gas.
Use: For the cultivation of Sulfuricurvum kujiense.