M-Brilliant Green Broth

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

M-Brilliant Green Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone.........................................................................20.0g
Lactose........................................................................................20.0g
Saccharose ..................................................................................20.0g
NaCl............................................................................................10.0g
Yeast extract..................................................................................6.0g
Phenol Red..................................................................................0.16g
Brilliant Green..........................................................................0.025g
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to1.0L. Mix thoroughly. Distribute into tubes
containing inverted Durham tubes, in 10.0mL amounts for testing
1.0mL or less of sample. Gently heat and bring to boiling. Do not au-
toclave. Cool the broth rapidly. Medium is sensitive to light.
Use: For the detection of coliform microorganisms in foods, dairy
products, water, and wastewater, as well as in other materials of sani-
tary importance.