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McBride Listeria HiVeg Agar Base, Modified, with Blood and Selective Supplement (Modified McBride Listeria HiVeg Agar Base)
[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]
McBride Listeria HiVeg Agar Base, Modified,
with Blood and Selective Supplement
(Modified McBride Listeria HiVeg Agar Base)
Glycine anhydride.......................................................................10.0g
NaCl..............................................................................................5.0g
Plant extract ..................................................................................3.0g
Phenyl ethanol...............................................................................2.5g
LiCl...............................................................................................0.5g
Sheep blood, defibrinated........................................................50.0mL
Selective supplement solution .................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium, without blood or selective supplement solu-
tion, is available as a premixed powder from HiMedia.
Caution: LiCl is harmful. Avoid bodily contact and inhalation of va-
pors. On contact with skin wash with plenty of water immediately.
Selective Supplement Solution:
Composition per 10.0mL:
Cycloheximide..............................................................................0.2g
Preparation of Selective Supplement Solution: Add cyclohex-
imide to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except blood and se-
lective supplement solution, to distilled/deionized water and bring vol-
ume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add 50.0mL defibrinated blood and
10.0mL selective supplement solution. Mix thoroughly. Pour into ster-
ile Petri dishes or leave in tubes.
Use: For the selective isolation of Listeria monocytogenes from clini-
cal and nonclinical specimens containing mixed flora.
with Blood and Selective Supplement
(Modified McBride Listeria HiVeg Agar Base)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ............................................................................................15.0gGlycine anhydride.......................................................................10.0g
NaCl..............................................................................................5.0g
Plant extract ..................................................................................3.0g
Phenyl ethanol...............................................................................2.5g
LiCl...............................................................................................0.5g
Sheep blood, defibrinated........................................................50.0mL
Selective supplement solution .................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium, without blood or selective supplement solu-
tion, is available as a premixed powder from HiMedia.
Caution: LiCl is harmful. Avoid bodily contact and inhalation of va-
pors. On contact with skin wash with plenty of water immediately.
Selective Supplement Solution:
Composition per 10.0mL:
Cycloheximide..............................................................................0.2g
Preparation of Selective Supplement Solution: Add cyclohex-
imide to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except blood and se-
lective supplement solution, to distilled/deionized water and bring vol-
ume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add 50.0mL defibrinated blood and
10.0mL selective supplement solution. Mix thoroughly. Pour into ster-
ile Petri dishes or leave in tubes.
Use: For the selective isolation of Listeria monocytogenes from clini-
cal and nonclinical specimens containing mixed flora.
