McCarthy Agar

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

McCarthy Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Cornstarch...................................................................................10.0g
Naladixic acid...........................................................................0.015g
Colistin........................................................................................0.01g
GC agar base.................................................................................1.0L
pH 7.2 ± 0.2 at 25°C
GC Agar Base:
Composition per liter:
Agar ............................................................................................10.0g
Pancreatic digest of casein............................................................7.5g
Peptic digest of animal tissue........................................................7.5g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Cornstarch.....................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Preparation of GC Agar Base: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: To 1.0L of GC agar base, add the corn-
starch. Gently heat while stirring to dissolve. Add the naladixic acid
and colistin. Mix thoroughly. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the isolation and differentiation of Gardnerella vaginalis
(Haemophilus vaginalis, Corynebacterium vaginale) from genitouri-
nary specimens. Bacteria that can utilize starch appear as colonies sur-
rounded by a clear zone.