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McClung-Toabe Egg Yolk Agar, CDC Modified (CDC Modified McClung-Toabe Egg Yolk Agar)

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

McClung-Toabe Egg Yolk Agar, CDC Modified
(CDC Modified McClung-Toabe Egg Yolk Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................40.0g
Agar ............................................................................................25.0g
NaHPO 4 ........................................................................................5.0g
Yeast extract..................................................................................5.0g
D- Glucose......................................................................................2.0g
NaCl..............................................................................................2.0g
Egg yolk emulsion.................................................................100.0mL
MgSO 4 (5% solution) ................................................................0.2mL
pH 7.4 ± 0.2 at 25°C
Egg Yolk Emulsion:
Composition :
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
Preparation of Egg Yolk Emulsion: Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs. Sep-
arate yolks from whites for 11 eggs. Mix egg yolks with 1 chicken egg.
Preparation of Medium: Add components, except egg yolk emul-
sion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat while stirring and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 60°C. Aseptically add
100.0mL of sterile egg yolk emulsion. Mix thoroughly. Pour into ster-
ile Petri dishes in 20.0mL volumes.
Use: For the isolation, cultivation, and differentiation of anaerobic
bacteria from foods. Bacteria that produce lecithinase appear as colo-
nies surrounded by an insoluble opaque precipitate. Bacteria that pro-
duce lipase activity appear as colonies with a sheen or “pearly” surface.
Bacteria that possess proteolytic activity appear as colonies surrounded
by a clear zone.

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McClung-Toabe Egg Yolk Agar, CDC Modified (CDC Modified McClung-Toabe Egg Yolk Agar)