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McClung-Toabe Egg Yolk Agar, CDC Modified (CDC Modified McClung-Toabe Egg Yolk Agar)
[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]
McClung-Toabe Egg Yolk Agar, CDC Modified
(CDC Modified McClung-Toabe Egg Yolk Agar)
Agar ............................................................................................25.0g
Na 2 HPO 4 .......................................................................................5.0g
Yeast extract..................................................................................5.0g
Glucose .........................................................................................2.0g
NaCl..............................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
Egg yolk emulsion, 50%........................................................100.0mL
MgSO 4 ·7H 2 O (5% solution)......................................................0.2mL
pH 7.3 ± 0.2 at 25°C
Egg Yolk Emulsion, 50%:
Composition per 100.0mL:
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
NaCl (0.9% solution)...............................................................50.0mL
Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Beat to form emulsion. Measure 50.0mL of egg yolk emulsion and add
to 50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize.
Warm to 45°–50°C.
Preparation of Medium: Add components—except egg yolk
emulsion, 50%—to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Gently heat while stirring and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 100.0mL of sterile egg yolk emulsion, 50%. Mix thor-
oughly. Pour into sterile Petri dishes in 15.0mL volumes.
Use: For the cultivation of a wide variety of anaerobic bacteria. For the
differentiation of anaerobic bacteria based on lecithinase production,
lipase production, and proteolytic ability. Bacteria that produce lecithi-
nase appear as colonies surrounded by a zone of insoluble precipitate.
Bacteria that produce lipase appear as colonies with a pearly iridescent
sheen. Bacteria that produce proteolytic activity appear as colonies sur-
rounded by a clear zone.
(CDC Modified McClung-Toabe Egg Yolk Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Pancreatic digest of casein..........................................................40.0gAgar ............................................................................................25.0g
Na 2 HPO 4 .......................................................................................5.0g
Yeast extract..................................................................................5.0g
Glucose .........................................................................................2.0g
NaCl..............................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
Egg yolk emulsion, 50%........................................................100.0mL
MgSO 4 ·7H 2 O (5% solution)......................................................0.2mL
pH 7.3 ± 0.2 at 25°C
Egg Yolk Emulsion, 50%:
Composition per 100.0mL:
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
NaCl (0.9% solution)...............................................................50.0mL
Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Beat to form emulsion. Measure 50.0mL of egg yolk emulsion and add
to 50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize.
Warm to 45°–50°C.
Preparation of Medium: Add components—except egg yolk
emulsion, 50%—to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Gently heat while stirring and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 100.0mL of sterile egg yolk emulsion, 50%. Mix thor-
oughly. Pour into sterile Petri dishes in 15.0mL volumes.
Use: For the cultivation of a wide variety of anaerobic bacteria. For the
differentiation of anaerobic bacteria based on lecithinase production,
lipase production, and proteolytic ability. Bacteria that produce lecithi-
nase appear as colonies surrounded by a zone of insoluble precipitate.
Bacteria that produce lipase appear as colonies with a pearly iridescent
sheen. Bacteria that produce proteolytic activity appear as colonies sur-
rounded by a clear zone.
