M-CP Agar Base

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

M-CP Agar Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose......................................................................................30.0g
Yeast extract................................................................................20.0g
Agar............................................................................................15.0g
Sucrose..........................................................................................5.0g
L -Cysteine·HCl·H 2 O.....................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
FeCl 3 ·6H 2 O.................................................................................0.09g
Indoxyl β- D -glucoside ................................................................0.06g
Bromcresol Purple ......................................................................0.04g
Selective supplement solution B..............................................20.0mL
Selective supplement solution A..............................................10.0mL
pH 7.6 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution A:
Composition per 10.0mL:
D -Cycloserine.........................................................................400.0mg
Polymyxin B sulfate.................................................................25.0mg
Preparation of Selective Supplement Solution A:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Selective Supplement Solution B:
Composition per 20.0mL:
Phenolphthalein diphosphate........................................................0.1g
Preparation of Selective Supplement Solution B:  Add phenol-
phthalein diphosphate to distilled/deionized water and bring volume to
20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solutions A and B, to distilled/deionized water and bring vol-
ume to 970.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add selective supplement
solutions A and B. Mix thoroughly. Pour into Petri dishes or aseptically
distribute into sterile tubes.
Use: Recommended by the Directive of the Council of the European
Union 98/83/EC for the isolation and enumeration of Clostridium per-
fringens from water samples using the membrane filtration technique.