M-CP HiVeg Agar Base with Phenolphthalein Diphosphate

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

M-CP HiVeg Agar Base
with Phenolphthalein Diphosphate

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate No. 1...............................................................30.0g
Agar ............................................................................................15.0g
L -Cysteine·HCl..............................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
FeCl 3 ·6H 2 O...................................................................................0.09
Indoxyl β- D -glucoside...................................................................0.06
Bromcresol Purple ........................................................................0.04
Sucrose..........................................................................................5.0g
Yeast extract................................................................................20.0g
Phenolphthalein diphosphate solution.....................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without phenolphthalein diphosphate, is avail-
able as a premixed powder from HiMedia.
Phenolphthalein Diphosphate Solution:
Composition per 10.0mL:
Phenolphthalein diphosphate........................................................2.0g
Preparation of Phenolphthalein Diphosphate Solution: Add
phenolphthalein diphosphate to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except phenolphtha-
lein diphosphate solution, to distilled/deionized water and bring vol-
ume to 990.0mL. Mix thoroughly. Gently heat while stirring until
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45-
50°C. Aseptially add 10.0mL of sterile phenolphthalein diphosphate
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation and identification of Providencia stuartii.