m-CP Medium

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

m-CP Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose......................................................................................30.0g
Yeast extract................................................................................20.0g
Agar............................................................................................15.0g
Sucrose..........................................................................................5.0g
L -Cysteine·HCl·H 2 O.....................................................................1.0g
MgO 4 ·7H 2 O..................................................................................0.1g
Bromcresol Purple......................................................................0.04g
Phenolphthalein biphosphate tetrazolium
salt solution .......................................................................10.0mL
Selective supplement solution ...................................................4.0mL
Indoxyl-β- D -glucoside solution.................................................4.0mL
Ferric chloride solution..............................................................1.0mL
pH 7.6 ± 0.2 at 25°C
Selective Supplement Solution:
Composition per 4.0mL :
D -Cycloserine................................................................................0.4g
Polymyxin B sulfate ................................................................25.0mg
Preparation of Selective Supplement Solution: Add compo-
nents to 4.0mL of distilled/deionized water. Mix thoroughly. Filter ster-
ilize.
Phenolphthalein Biphosphate Tetrazolium
Salt Solution:
Composition per 10.0mL :
Phenolphthalein biphosphate tetrazolium
salt .....................................................................................25.0mg
Preparation of Phenolphthalein Biphosphate Tetrazolium
Salt Solution: Add phenolphthalein biphosphate tetrazolium salt to
10.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.
Indoxyl- β - D -glucoside Solution:
Composition per 10.0mL :
Indoxyl-β- D -glucoside................................................................0.45g
Preparation of Indoxyl- β - D -glucoside Solution: Add indoxyl-
β- D -glucoside to 10.0mL of distilled/deionized water. Mix thoroughly.
Filter sterilize.
Ferric Chloride Solution:
Composition per 4.0mL :
FeCl 3 ·6H 2 O..............................................................................30.0mg
Preparation of Ferric Chloride Solution: Add FeCl 3 ·6H 2 O to
4.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, phenolphthalein biphosphate tetrazolium salt solu-
tion, indoxyl-β- D -glucoside solution, and ferric chloride solution, to
distilled/deionized water and bring volume to 981.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 4.0mL of selective supplement solution. Mix thor-
oughly. Aseptically add 10.0mL phenolphthalein biphosphate tetrazo-
lium salt solution, 4.0mL indoxyl-β- D -glucoside solution, and 1.0mL
ferric chloride solution. Mix thoroughly. Pour into sterile Petri dishes or
aseptically distribute into tubes.
Use:  A selective, chromogenic medium for the rapid identification and
enumeration of Clostridium perfringens in water samples, including
water used in food and beverage production.