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MCP Medium (Modified MacConkey Medium) (MacConkey Phosphatase Medium)
[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]
MCP Medium
(Modified MacConkey Medium)
(MacConkey Phosphatase Medium)
Agar ............................................................................................13.5g
Lactose........................................................................................10.0g
NaCl..............................................................................................5.0g
Bile salts........................................................................................1.5g
Pancreatic digest of casein............................................................1.5g
Peptic digest of animal tissue........................................................1.5g
Na 2 HPO 4 .......................................................................................0.6g
Glucose .........................................................................................0.2g
Methyl Blue ................................................................................0.07g
Neutral Red.................................................................................0.03g
Crystal Violet.............................................................................1.0mg
Phenolphthalein diphosphate solution.....................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without phenolphthalein diphosphate, is avail-
able as a premixed powder from HiMedia.
Phenolphthalein Diphosphate Solution:
Composition per 10.0mL:
Phenolphthalein diphosphate........................................................2.0g
Preparation of Phenolphthalein Diphosphate Solution: Add
phenolphthalein diphosphate to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except phenolphtha-
lein diphosphate solution, to distilled/deionized water and bring vol-
ume to 990.0mL. Mix thoroughly. Gently heat while stirring until
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45-
50°C. Aseptially add 10.0mL of sterile phenolphthalein diphosphate
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation and identification of Providencia stuartii.
(Modified MacConkey Medium)
(MacConkey Phosphatase Medium)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Pancreatic digest of gelatin.........................................................17.0gAgar ............................................................................................13.5g
Lactose........................................................................................10.0g
NaCl..............................................................................................5.0g
Bile salts........................................................................................1.5g
Pancreatic digest of casein............................................................1.5g
Peptic digest of animal tissue........................................................1.5g
Na 2 HPO 4 .......................................................................................0.6g
Glucose .........................................................................................0.2g
Methyl Blue ................................................................................0.07g
Neutral Red.................................................................................0.03g
Crystal Violet.............................................................................1.0mg
Phenolphthalein diphosphate solution.....................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without phenolphthalein diphosphate, is avail-
able as a premixed powder from HiMedia.
Phenolphthalein Diphosphate Solution:
Composition per 10.0mL:
Phenolphthalein diphosphate........................................................2.0g
Preparation of Phenolphthalein Diphosphate Solution: Add
phenolphthalein diphosphate to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except phenolphtha-
lein diphosphate solution, to distilled/deionized water and bring vol-
ume to 990.0mL. Mix thoroughly. Gently heat while stirring until
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45-
50°C. Aseptially add 10.0mL of sterile phenolphthalein diphosphate
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation and identification of Providencia stuartii.
