MD Medium

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

MD Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
L- Malic acid.................................................................................20.0g
Pancreatic digest of casein..........................................................10.0g
D- Glucose......................................................................................5.0g
Casamino acids.............................................................................3.0g
Pancreatic digest of soybean meal................................................1.5g
Tween™ 80...................................................................................1.0g
Yeast extract..................................................................................1.0g
Bromcresol Green solution......................................................20.0mL
pH 7.0 ± 0.2 at 25°C
Bromcresol Green Solution:
Composition per 30.0mL:
Bromcresol Green.........................................................................0.1g
NaOH (0.01N solution)............................................................30.0mL
Preparation of Bromcresol Green Solution: Add Bromcresol
Green to 30.0mL of NaOH solution. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Adjust pH to 7.0 with 10N
KOH. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the isolation and cultivation of Salmonella species from
foods.