MD 1 Medium

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

MD 1 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................3.0g
MgSO 4 ·7H 2 O................................................................................2.0g
CaCl 2 .............................................................................................0.5g
Trace elements solution.............................................................1.0mL
Vitamin B 12 solution..................................................................1.0mL
Trace Elements Solution:
Composition per liter:
EDTA............................................................................................8.0g
MnCl 2 ·4H 2 O .................................................................................0.1g
CoCl 2 ..........................................................................................0.02g
KBr .............................................................................................0.02g
ZnCl 2 ...........................................................................................0.02g
CuSO 4 .........................................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
NaMoO 4 ·2H 2 O............................................................................0.01g
BaCl 2 ..........................................................................................5.0mg
LiCl............................................................................................5.0mg
SnCl 2 ·2H 2 O................................................................................5.0mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin B 12 Solution:
Composition per 10.0mL:
Vitamin B 12 ................................................................................5.0mg
Preparation of Vitamin B 12 Solution: Add vitamin B 12 to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of myxobacteria.