MD1- Medium (DSMZ Medium 1118)

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

MD1- Medium
(DSMZ Medium 1118)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casitone .......................................................................................3.0g
MgSO 4 · 7H 2 O................................................................................2.0g
CaCl 2 ·2H 2 O ..................................................................................0.7g
Vitamin solution ......................................................................10.0mL
Trace elements solution SL-4 ...................................................1.0mL
pH 7.1 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O................................................................................. 0.2g
Trace elements solution SL-6................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Vitamin Solution:
Composition per 10.0ml:
Vitamin B 12 ................................................................................0.5mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Adjust pH to 7.1. Distribute into tubes or flasks. Gently
heat while stirring and bring to boiling. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C. Aseptically add 10.0mL sterile vita-
min solution. Mix thorougly. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Myxococcus xanthus.