Medium AS4

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

Medium AS4

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................80.0g
PPLO broth without Crystal Violet........................................500.0mL
Horse serum...........................................................................200.0mL
Phenol Red (0.5% solution).......................................................5.0mL
pH 7.2 ± 0.2 at 25°C
PPLO Broth without Crystal Violet:
Composition per 500.0mL:
Beef heart, solids from infusion................................................11.53g
Peptone........................................................................................2.33g
NaCl............................................................................................1.15g
Source: PPLO broth without Crystal Violet is available as a premixed
powder from BD Diagnostic Systems.
Preparation of PPLO Broth without Crystal Violet:  Add
components to distilled/deionized water and bring volume to 500.0mL.
Mix thoroughly. Beef heart for infusion may be substituted; 100.0g of
beef heart for infusion is equivalent to 500.0g of fresh heart tissue.
Preparation of Medium: Add components, except horse serum, to
distilled/deionized water and bring volume to 800.0mL. Mix thorough-
ly. Adjust pH to 7.2. Autoclave for 10 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add 200.0mL of noninactivated, sterile
horse serum. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use:  For the cultivation and maintenance of Spiroplasma melliferum.