Medium for Aciduric, Thermophilic Bacillus Strains

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Aciduric, Thermophilic Bacillus Strains

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................500.0mL
Solution B..............................................................................500.0mL
pH 4.3 ± 0.2 at 25°C
Solution A:
Composition per 500.0mL:
KH 2 PO 4 .........................................................................................3.0g
Glucose .........................................................................................1.0g
Starch ............................................................................................1.0g
Tryptone........................................................................................1.0g
Yeast extract..................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ·2H 2 O ................................................................................0.25g
(NH 4 ) 2 SO 4 ....................................................................................0.2g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 4.3.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Solution B:
Composition per 500.0mL:
Agar............................................................................................20.0g
Preparation of Solution B:  Add agar to distilled/deionized water
and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 50°–55°C.
Preparation of Medium:  Aseptically combine 500.0mL of solu-
tion A and 500.0mL of solution B. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the cultivation of aciduric, thermophilic Bacillus strains.