Medium for Ammonia-Oxidizing Bacteria

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Ammonia-Oxidizing Bacteria

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 .............................................................................235.0mg
KH 2 PO 4 ..................................................................................200.0mg
CaCl 2 ·2H 2 O .............................................................................40.0mg
MgSO 4 ·7H 2 O...........................................................................40.0mg
Iron-EDTA-Phenol Red solution...............................................1.0mL
Na 2 CO 3  solution...................................................................... variable
Na 2 CO 3 Solution:
Composition per 100.0mL:
Na 2 CO 3 .........................................................................................5.0g
Preparation of Na 2 CO 3 Solution:  Add Na 2 CO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C.
Iron-EDTA-Phenol Red Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O.............................................................................50.0mg
Sodium EDTA..........................................................................50.0mg
Phenol Red...............................................................................50.0mg
Preparation of Iron-EDTA-Phenol Red Solution: Add compo-
nents to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly.
Preparation of Medium: Add components, except Na 2 CO 3  solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Add enough sterile Na 2 CO 3  solution to turn the medi-
um pale pink. During incubation and growth of bacteria, add additional
sterile Na 2 CO 3  solution to restore the pale pink color. Growth is com-
plete when no further color change is observed.
Use: For the cultivation of Nitrosolobus multiformis and Nitrosomo-
nas europaea.