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培养基配方
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Medium B for Sulfate Reducers (Postgate’s Medium B for Sulfate Reducers)
[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]
Medium B for Sulfate Reducers
(Postgate’s Medium B for Sulfate Reducers)
MgSO 4 ·7H 2 O................................................................................2.0g
NH 4 Cl ...........................................................................................1.0g
CaSO 4 ...........................................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.5g
Ascorbic acid ................................................................................0.1g
Thioglycollic acid.........................................................................0.1g
pH 7.0–7.5 at 25°C
Preparation of Medium: Add components, except ascorbic acid
and thioglycollic acid, to tap water and bring volume to 1.0L. For ma-
rine bacteria, NaCl may be added or seawater used in place of tap wa-
ter. Mix thoroughly. Adjust pH to 7.0–7.5. Thioglycolate and ascorbate
should be added immediately prior to sterilization. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation, cultivation, and maintenance of Desulfovibrio
species and Desulfotomaculum species. This medium turns black as a
result of H 2 S production due to bacterial growth.
(Postgate’s Medium B for Sulfate Reducers)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Sodium lactate...............................................................................3.5gMgSO 4 ·7H 2 O................................................................................2.0g
NH 4 Cl ...........................................................................................1.0g
CaSO 4 ...........................................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.5g
Ascorbic acid ................................................................................0.1g
Thioglycollic acid.........................................................................0.1g
pH 7.0–7.5 at 25°C
Preparation of Medium: Add components, except ascorbic acid
and thioglycollic acid, to tap water and bring volume to 1.0L. For ma-
rine bacteria, NaCl may be added or seawater used in place of tap wa-
ter. Mix thoroughly. Adjust pH to 7.0–7.5. Thioglycolate and ascorbate
should be added immediately prior to sterilization. Distribute into
tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation, cultivation, and maintenance of Desulfovibrio
species and Desulfotomaculum species. This medium turns black as a
result of H 2 S production due to bacterial growth.
