Medium for Campylobacter DSM 806 (DSMZ Medium 121)

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Campylobacter DSM 806
(DSMZ Medium 121)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na-aspartate................................................................................10.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Yeast extract..................................................................................0.2g
CaCl 2 ·2H 2 O .............................................................................28.0mg
Resazurin...................................................................................1.0mg
Cysteine phosphate solution..................................................100.0mL
Trace elements solution SL-10..................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O...........................................................................190.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O.......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Gas under 80% N 2  + 20% CO 2 .
Cysteine Phosphate Solution:
Composition per 100.0mL:
K 2 HPO 4 .......................................................................................0.75g
NaH 2 PO 4 ...................................................................................0.25g
Cysteine-HCl·H 2 O......................................................................0.25g
Preparation of Cysteine Phosphate Solution : Add components
to 100.0mL distilled/deionized water. Mix thoroughly. Gas under 80%
N 2  + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except cysteine phos-
phate solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically add 100.0mL of sterile cysteine phosphate solution. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Sulfurospirillum sp.