Medium for Carbon Monoxide Oxidizers

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Carbon Monoxide Oxidizers

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................12.0g
Na 2 HPO 4 ·12H 2 O...........................................................................4.5g
NH 4 Cl ...........................................................................................1.5g
KH 2 PO 4 .......................................................................................0.75g
MgSO 4 ·7H 2 O............................................................................... 0.2g
CaCl 2 ·2H 2 O.................................................................................0.03g
Ferric ammonium citrate...........................................................0.018g
Trace elements solution SL-6 ....................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri dishes or distribute into sterile tubes. After inoculation, in-
cubate in an atmosphere of 80% CO +10% O 2 + 10% N 2 .
Use: For the chemoautotrophic cultivation and maintenance of Alcal-
igenes species, Pseudomonas carboxydohydrogena, and other Pseudo-
monas species.