Medium E-2

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium E-2

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

K 2 HPO 4 ·3H 2 O ..............................................................................7.5g
KH 2 PO 4 .........................................................................................3.7g
NaNH 4 HPO 4 ·4H 2 O.......................................................................3.5g
Tap water ......................................................................................1.0L
Thiamine solution....................................................................10.0mL
MgSO 4 ·7H 2 O solution.............................................................10.0mL
n-Octane.................................................................................. variable
pH 7.0 ± 0.2 at 25°C
Thiamine Solution:
Composition per 10.0mL:
Thiamine..................................................................................10.0mg
Preparation of Thiamine Solution:  Add thiamine to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly.
MgSO 4 ·7H 2 O Solution:
Composition per 10.0mL:
MgSO 4 ·7H 2 O............................................................................0.246g
Preparation of MgSO 4 ·7H 2 O Solution:  Add MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly.
Preparation of Medium: Add components, except octane, to tap
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Inoculate tubes and place in a desiccator to which n-octane has
been added and evaporated.
Use: For the cultivation of a recombinant strain of Escherichia coli
that utilizes hydrocarbons.