Medium for Ectothiorhodospira

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Ectothiorhodospira

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Basal medium ........................................................................800.0mL
Solution C..............................................................................200.0mL
Vitamin solution B.....................................................................1.0mL
Basal Medium:
Composition per 800.0mL:
NaCl..........................................................................................180.0g
Na 2 SO 4 ........................................................................................20.0g
Na 2 CO 3 .........................................................................................6.0g
Na 2 S·9H 2 O....................................................................................1.0g
Sodium succinate..........................................................................1.0g
NH 4 Cl ...........................................................................................0.8g
KH 2 PO 4 .........................................................................................0.5g
Yeast extract..................................................................................0.5g
MgCl 2 ·6H 2 O..................................................................................0.1g
CaCl 2 ·7H 2 O.................................................................................0.05g
Trace elements solution A..........................................................1.0mL
pH 8.5 ± 0.2 at 25°C
Trace Elements Solution A:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.8g
H 3 BO 3 ....................................................................................500.0mg
CoCl 2 ·6H 2 O ...........................................................................250.0mg
ZnCl 2 ......................................................................................100.0mg
MnCl 2 ·4H 2 O.............................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................30.0mg
CuCl 2 ·2H 2 O .............................................................................10.0mg
NiCl 2 ·6H 2 O..............................................................................10.0mg
Na 2 SeO 3 ·5H 2 O.........................................................................10.0mg
Preparation of Trace Elements Solution A: Add components to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Adjust pH to 3 with 1N HCl. Bring volume to 1.0L with distilled/
deionized water.
Preparation of Basal Solution: Add components to distilled/de-
ionized water and bring volume to 800.0mL. Mix thoroughly. Adjust
pH to 8.5. Distribute into screw-capped bottles. Autoclave for 15 min
at 14 psi pressure–120°C.
Vitamin Solution B:
Composition per 100.0mL:
Nicotinamide............................................................................35.0mg
Thiamine dichloride.................................................................30.0mg
p-Aminobenzoic acid...............................................................20.0mg
Biotin .......................................................................................10.0mg
Calcium  DL -pantothenate.........................................................10.0mg
Pyridoxal·HCl..........................................................................10.0mg
Vitamin B 12 ................................................................................5.0mg
Preparation of Vitamin Solution B: Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Solution C:
Composition per 200.0mL:
NaHCO 3 ......................................................................................14.0g
Preparation of Solution C: Add NaHCO 3 to distilled/deionized
water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: To 800.0mL of sterile basal solution,
aseptically add 200.0mL of sterile solution C and 1.0mL of sterile vi-
tamin solution B. Mix thoroughly.
Use:  For the cultivation and maintenance of Ectothiorhodospira hal-
ochloris.