Medium with EDTA as Carbon and Nitrogen Source

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium with EDTA as Carbon and Nitrogen Source

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
MgSO 4 ·7H 2 O................................................................................0.3g
Disodium ethylenediaminetetraacetate.......................................0.25g
CaCl 2 ·2H 2 O ..............................................................................0.244g
Ferric ammonium citrate.............................................................0.05g
Phosphate solution...................................................................50.0mL
Trace elements solution SL-6....................................................5.0mL
Schlegel’s vitamin solution........................................................5.0mL
pH 7.6 ± 0.4 at 25°C
Phosphate Solution:
Composition per 50.0mL:
Na 2 HPO 4 ·2H 2 O...........................................................................3.57g
KH 2 PO 4 .......................................................................................0.67g
Preparation of Phosphate Solution : Add components to dis-
tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.
Adjust pH to 7.6. Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6 : Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Schlegel’s Vitamin Solution:
Composition per 100.0mL:
Nicotinic acid................................................................................2.0g
Pyridoxamine.............................................................................5.0mg
Cyanocobalamin........................................................................2.0mg
p-Aminobenzoate.......................................................................1.0mg
Thiamine....................................................................................1.0mg
Calcium  DL -pantothenate...........................................................0.5mg
Biotin.........................................................................................0.2mg
Preparation of Schlegel’s Vitamin Solution : Add components
to distilled/deionized water and bring volume to 100.0mL. Filter ster-
ilize.
Preparation of Medium: Add components, except phosphate solu-
tion and Schlegel’s vitamin solution, to distilled/deionized water and
bring volume to 945.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically
add 50.0mL of sterile phosphate solution and 5.0mL of sterile Schle-
gel’s vitamin solution. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation of bacteria that can utilize EDTA as a carbon
source.