Medium F

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium F

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgSO 4 ·7H 2 O................................................................................0.5g
(NH 4 ) 2 SO 4 ..................................................................................0.15g
KCl..............................................................................................0.05g
KH 2 PO 4 .......................................................................................0.05g
Ca(NO 3 ) 2 .....................................................................................0.01g
FeSO 4 ·7H 2 O solution...............................................................10.0mL
pH 3.5 ± 0.2 at 25°C
FeSO 4 ·7H 2 O Solution:
Composition per 10.0mL:
FeSO 4 ·7H 2 O..................................................................................1.0g
Preparation of FeSO 4 ·7H 2 O Solution: Add the FeSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except FeSO 4 ·7H 2 O
solution, to tap water and bring volume to 990.0mL. Mix thoroughly.
Gently heat until dissolved. Adjust pH to 3.5. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile FeSO 4 ·7H 2 O solution. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation of Thiobacillus species.