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Medium F for Sulfate Reducers (Postgate’s Medium F for Sulfate Reducers)
[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]
Medium F for Sulfate Reducers
(Postgate’s Medium F for Sulfate Reducers)
Pancreatic digest of casein..........................................................10.0g
Sodium lactate ..............................................................................3.5g
Ferrous citrate...............................................................................0.5g
Na 2 SO 3 ..........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
Ascorbic acid................................................................................0.1g
Sodium thioglycolate....................................................................0.1g
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components, except ascorbic acid
and thioglycollic acid, to tap water and bring volume to 1.0L. For ma-
rine bacteria, NaCl may be added or seawater used in place of tap wa-
ter. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.1.
Thioglycolate and ascorbate should be added immediately prior to ster-
ilization. Distribute into screw-capped tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For isolation and cultivation of Desulfotomaculum nigrificans,
Desulfovibrio species, and other Desulfotomaculum species especially
in food. These bacteria form black colonies in deep agar cultures.
(Postgate’s Medium F for Sulfate Reducers)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar............................................................................................12.0gPancreatic digest of casein..........................................................10.0g
Sodium lactate ..............................................................................3.5g
Ferrous citrate...............................................................................0.5g
Na 2 SO 3 ..........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
Ascorbic acid................................................................................0.1g
Sodium thioglycolate....................................................................0.1g
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components, except ascorbic acid
and thioglycollic acid, to tap water and bring volume to 1.0L. For ma-
rine bacteria, NaCl may be added or seawater used in place of tap wa-
ter. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.1.
Thioglycolate and ascorbate should be added immediately prior to ster-
ilization. Distribute into screw-capped tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For isolation and cultivation of Desulfotomaculum nigrificans,
Desulfovibrio species, and other Desulfotomaculum species especially
in food. These bacteria form black colonies in deep agar cultures.
