Medium for Freshwater Flexibacteria

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Freshwater Flexibacteria

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1002.0mL:

Casamino acids.............................................................................1.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Tris (hydroxymethyl) amino methane...........................................1.0g
CaCl 2 ·2H 2 O...................................................................................0.1g
KNO 3 ............................................................................................0.1g
Sodium glycerophosphate.............................................................0.1g
Thiamine....................................................................................1.0mg
Cobalamine.................................................................................1.0μg
Glucose solution ........................................................................1.0mL
Trace elements solution .............................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose .......................................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Trace Elements Solution:
Composition per liter:
ZnCl 2 ...........................................................................................20.8g
H 3 BO 3 .........................................................................................2.85g
MnCl 2 ·4H 2 O..................................................................................1.8g
Sodium tartrate............................................................................1.77g
FeSO 4 ..........................................................................................1.36g
CoCl 2 ·6H 2 O .............................................................................40.4mg
CuCl 2 ·2H 2 O .............................................................................26.9mg
Na 2 MoO 4 ·2H 2 O .......................................................................25.2mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except glucose solu-
tion and trace elements solution, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pres-
sure–121°C. Aseptically add 1.0mL of sterile glucose solution and
1.0mL of sterile trace elements solution. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation of Cytophaga psychrophila, Flectobacillus
major, Flexibacter aurantiacus, Flexibacter aurantiacus, Flexibacter ele-
gans, Flexibacter flexilis, Flexibacter roseolus, Flexibacter ruber, Flexi-
bacter sancti, and Herpetosiphon geysericola.