Medium G for Sulfate Reducers (Postgate’s Medium G for Sulfate Reducers)

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium G for Sulfate Reducers
(Postgate’s Medium G for Sulfate Reducers)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1015.2mL:

Solution 1...............................................................................970.0mL
Solution 4.................................................................................30.0mL
Solution 8A, 8B, 8C, 8D, or 8E...............................................10.0mL
Solution 5...................................................................................3.0mL
Solution 2...................................................................................1.0mL
Solution 3...................................................................................1.0mL
Solution 6...................................................................................0.1mL
Solution 7...................................................................................0.1mL
pH 7.2 ± 0.2 at 25°C
Solution 1:
Composition per 970.0mL:
Na 2 SO 4 ..........................................................................................3.0g
NaCl..............................................................................................1.2g
MgCl 2 ·6H 2 O..................................................................................0.4g
KCl................................................................................................0.3g
NH 4 Cl ...........................................................................................0.3g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.15g
Preparation of Solution 1:  Add components to distilled/deionized
water and bring volume to 970.0mL. Mix thoroughly. Adjust pH to 7.2
with 2N HCl. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C.
Solution 2:
Composition per 10.0mL:
NaOH.........................................................................................5.0mg
Na 2 SeO 3 ...................................................................................0.03mg
Preparation of Solution 2:  Add NaOH and Na 2 SeO 3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Solution 3:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ................................................................................0.12g
MnCl 2 ·4H 2 O..................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .........................................................................................0.06g
NiCl 2 ·6H 2 O...............................................................................0.025g
NaMoO 4 ·2H 2 O..........................................................................0.025g
CuCl 2 ·2H 2 O ..............................................................................0.015g
Preparation of Solution 3:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 25°C.
Solution 4:
Composition per 30.0mL:
NaHCO 3 ......................................................................................2.55g
Preparation of Solution 4:  Add NaHCO 3 to distilled/deionized
water and bring volume to 30.0mL. Mix thoroughly. Gas with 100%
CO 2 for 10–15 min. Filter sterilize.
Solution 5:
Composition per 3.0mL:
Na 2 S·9H 2 O..................................................................................0.36g
Preparation of Solution 5:  Add Na 2 S·9H 2 O to distilled/deionized
water and bring volume to 3.0mL. Mix thoroughly. Gas with 100% N 2
for 5–10 min. Cap tube with a rubber stopper. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C.
Solution 6:
Composition per 100.0mL:
Thiamine·HCl .............................................................................0.01g
Cyanocobalamin ........................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Biotin .........................................................................................1.0mg
Preparation of Solution 6:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Solution 7:
Composition per 100.0mL:
Succinic acid.................................................................................0.6g
Isobutyric acid ..............................................................................0.5g
Valeric acid ...................................................................................0.5g
2-Methylbutyric acid ....................................................................0.5g
3-Methylbutyric acid ....................................................................0.5g
Caproic acid..................................................................................0.2g
Preparation of Solution 7:  Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 9.0
with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C.
Solution 8A:
Composition per 100.0mL:
Sodium acetate·3H 2 O..................................................................20.0g
Solution 8B:
Composition per 100.0mL:
Propionic acid...............................................................................7.0g
Solution 8C:
Composition per 100.0mL:
n-Butyric acid ...............................................................................8.0g
Solution 8D:
Composition per 100.0mL:
Benzoic acid..................................................................................5.0g
Solution 8E:
Composition per 100.0mL:
n-Palmitic acid..............................................................................5.0g
Preparation of Solutions 8A–E:  Add the appropriate amount of
component to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Adjust pH to 9.0 with NaOH. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: To 970.0mL of cooled, sterile solution 1,
aseptically add 1.0mL of sterile solution 2, 1.0mL of sterile solution 3,
30.0mL of sterile solution 4, 3.0mL of sterile solution 5, 0.1mL of ster-
ile solution 6, 0.1mL of sterile solution 7, and 10.0mL of sterile solu-
tion 8A, 8B, 8C, 8D, or 8E. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use:  For the isolation and cultivation of Desulfovibrio baarsii, Desul-
fovibrio sapovorans, Desulfobacter species, Desulfonema species,
Desulfobulbus species, and Desulfotomaculum acetoxidans.