Medium for Halophilic Archaea (DSMZ Medium 1184)

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Halophilic Archaea
(DSMZ Medium 1184)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl .........................................................................................195.0g
MgSO 4 · 7H 2 O..............................................................................50.8g
MgCl 2 ·6H 2 O ...............................................................................32.5g
Yeast extract .................................................................................5.0g
KCl ...............................................................................................5.0g
CaCl 2 ·2H 2 O ..................................................................................0.8g
NaBr .............................................................................................0.6g
NaHCO 3 ......................................................................................0.16g
pH 6.7 ± 0.3 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5–7.0.
Distribute into tubes or flasks. Gently heat while stirring and bring to
boiling. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of halophilic archaea. For the cultivation of
Pycnoporus cinnabarinus and Natrinema ejinorense.