Medium for Marine Flexibacteria

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Marine Flexibacteria

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
Tris (hydroxymethyl) amino methane ..........................................1.0g
KNO 3 ............................................................................................0.5g
Sodium glycerophosphate.............................................................0.1g
Trace elements solution.............................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
ZnCl 2 ...........................................................................................20.8g
H 3 BO 3 .........................................................................................2.85g
MnCl 2 ·4H 2 O .................................................................................1.8g
Sodium tartrate............................................................................1.77g
FeSO 4 ..........................................................................................1.36g
CoCl 2 ·6H 2 O.............................................................................40.4mg
CuCl 2 ·2H 2 O.............................................................................26.9mg
Na 2 MoO 4 ·2H 2 O.......................................................................25.2mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except trace elements
solution, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical-
ly add 1.0mL of sterile trace elements solution. Mix thoroughly. Asep-
tically distribute into sterile tubes or flasks.
Use: For the cultivation of Cytophaga aprica, Cytophaga diffluens,
Cytophaga johnsonae, Cytophaga lytica, Cytophaga species, Flexi-
bacter aggregans, Flexibacter aurantiacus, Flexibacter litoralis, Flexi-
bacter tractuosus, Flexithrix dorotheae, Herpetosiphon cohaerens, Her-
petosiphon nigricans, Herpetosiphon persicus, Microscilla arenaria,
Microscilla furvescens, Microscilla marina, Microscilla sericea, and
Saprospira grandis.