Medium for Marine Methylotrophs (DSMZ Medium 750)

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Marine Methylotrophs
(DSMZ Medium 750)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................25.0g
Agar............................................................................................20.0g
Peptone .......................................................................................10.0g
Beef extract...................................................................................7.0g
K 2 HPO 4 .........................................................................................1.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
Methanol..................................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 10.0mL filter sterilized
methanol. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the cultivation and maintenance of Methylophaga marina and
Methylophaga thalassica.