Medium for Methylobacterium podarium (DSMZ Medium 1032)

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Methylobacterium podarium
(DSMZ Medium 1032)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Na 2 HPO 4 · 2H 2 O.............................................................................7.9g
KH 2 PO 4 .........................................................................................1.5g
NH 4 Cl ...........................................................................................0.8g
MgSO 4 · 7H 2 O................................................................................0.1g
Methylamine solution..............................................................30.0mL
Trace metal solution (Kelly solution T)...................................10.0mL
pH 7.3 ± 0.2 at 25°C
Methylamine Solution:
Composition per 10.0ml:
Methylamine.................................................................................0.5g
Preparation of Methylamine Solution:  Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Trace Metal Solution (Kelly Solution T):
Composition per liter:
EDTA..........................................................................................50.0g
NaOH............................................................................................9.0g
CaCl 2 ·2H 2 O.................................................................................7.34g
FeSO 4 ·7H 2 O..................................................................................5.0g
MnCl 2 ·4H 2 O..................................................................................2.5g
ZnSO 4 ·7H 2 O.................................................................................1.0g
CoCl 2 ·6H 2 O ..................................................................................0.5g
(NH 4 ) 2 MoO 4 .................................................................................0.5g
CuSO 4 ·5H 2 O.................................................................................0.2g
Preparation of Trace Metal Solution: Add EDTA to 400.0mL
distilled/deionized water. Add NaOH with constant mixing. This is
best done in a 1–2L beaker on a magnetic stirrer. Add the other salts
individually to about 30-40mL water to dissolve before adding to the
EDTA-NaOH solution. Allow each component to mix thoroughly be-
fore adding the next component. Adjust pH to 6.0 using 1M NaOH (ap-
proximately 24.0mL). Bring volume to 1.0L with distilled/deionized
water. Filter sterilize. Do not autoclave! Store in a dark bottle.
Preparation of Medium: Add components, except trace metal so-
lution and methylamine solution, to distilled/deionized water and bring
volume to 960.0mL. Mix thoroughly. Adjust pH to 7.4. Gently heat
while stirring and bring to boiling. Mix thoroughly. Autoclave for 10
min at 105 psi pressure–115°C. Cool to 50°C. Aseptically add meth-
ylamine solution and trace metal solution. Mix thoroughly. Pour into
Petri dishes or aseptically distribute into sterile tubes.
Use: For the cultivation of Methylobacterium podarium.