Medium N

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Medium N

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................20.0g
Glucose .......................................................................................20.0g
Yeast nitrogen base without amino acids......................................6.7g
Casamino acids, vitamin free........................................................2.0g
Isoleucine......................................................................................0.1g
Valine............................................................................................0.1g
Deoxythymidine-5′-monophosphate solution .........................10.0mL
Deoxythymidine-5′-Monophosphate Solution:
Composition per 10.0mL:
Deoxythymidine-5′-monophosphate.......................................15.0mg
Preparation of Deoxythymidine-5′-Monophosphate Solution:
Add deoxythymidine-5′-monophosphate to distilled/deionized water
and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except deoxythymi-
dine-5′-monophosphate solution, to distilled/deionized water and
bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°C.
Aseptically add 10.0mL of sterile deoxythymidine-5′-monophosphate
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use:  For the cultivation and maintenance of Saccharomyces cerevi-
siae.