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培养基配方
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Medium N for Sulfate Reducers (Postgate’s Medium N for Sulfate Reducers)
[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]
Medium N for Sulfate Reducers
(Postgate’s Medium N for Sulfate Reducers)
Sodium lactate ..............................................................................6.0g
NH 4 Cl ...........................................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
Sodium citrate·2H 2 O.....................................................................0.3g
FeSO 4 ·7H 2 O..................................................................................0.1g
CaCl 2 ·6H 2 O ................................................................................0.06g
MgSO 4 ·7H 2 O..............................................................................0.06g
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. For marine bacteria, NaCl may be
added or seawater used in place of distilled/deionized water. Mix thor-
oughly. Adjust pH to 7.5. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For the detection, culturing, and storage of Desulfovibrio species
and many Desulfotomaculum species. This medium should be used
when a clear culture medium is desired such as for chemostat culture.
This medium may be cloudy after sterilization but usually clears on
cooling. It turns black as a result of H 2 S production due to bacterial
growth.
(Postgate’s Medium N for Sulfate Reducers)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
(NH 4 ) 2 SO 4 ....................................................................................7.0gSodium lactate ..............................................................................6.0g
NH 4 Cl ...........................................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
Sodium citrate·2H 2 O.....................................................................0.3g
FeSO 4 ·7H 2 O..................................................................................0.1g
CaCl 2 ·6H 2 O ................................................................................0.06g
MgSO 4 ·7H 2 O..............................................................................0.06g
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. For marine bacteria, NaCl may be
added or seawater used in place of distilled/deionized water. Mix thor-
oughly. Adjust pH to 7.5. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For the detection, culturing, and storage of Desulfovibrio species
and many Desulfotomaculum species. This medium should be used
when a clear culture medium is desired such as for chemostat culture.
This medium may be cloudy after sterilization but usually clears on
cooling. It turns black as a result of H 2 S production due to bacterial
growth.
