Medium for Prosthecomicrobium and Ancalomicrobium

[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Prosthecomicrobium
and Ancalomicrobium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 ..................................................................................0.25g
Glucose.......................................................................................0.25g
Na 2 HPO 4 ...................................................................................0.071g
Modified Hutner’s basal salts..................................................20.0mL
Vitamin solution.......................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Modified Hutner’s Basal Salts:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O ................................................................................3.34g
FeSO 4 ·7H 2 O..................................................................................0.1g
(NH 4 ) 2 MoO 4 ............................................................................9.25mg
Metals “44”..............................................................................50.0mL
Preparation of Modified Hutner’s Basal Salts: Add nitrilotria-
cetic acid to 500.0mL of distilled/deionized water. Dissolve by adjust-
ing pH to 6.5 with KOH. Add remaining components. Readjust pH to
7.2 with H 2 SO 4 or KOH. Add distilled/deionized water to 1.0L. Store
at 5°C.
Metals “44”:
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.................................................................................1.1g
FeSO 4 ·7H 2 O..................................................................................0.5g
EDTA..........................................................................................0.25g
MnSO 4 ·7H 2 O............................................................................0.154g
CuSO 4 ·5H 2 O...............................................................................0.04g
Co(NO 3 ) 2 ·6H 2 O........................................................................0.025g
Na 2 B 4 O 7 ·10H 2 O........................................................................0.018g
Preparation of Metals “44”: Add a few drops of H 2 SO 4 to dis-
tilled/deionized water to inhibit precipitate formation. Add compo-
nents to acidified distilled/deionized water and bring volume to
100.0mL. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Thiamine·HCl............................................................................5.0mg
D- Calcium pantothenate.............................................................5.0mg
Riboflavin..................................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-

ilize.

Preparation of Medium:  Add components, except vitamin solu-
tion, to distilled deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
room temperature. Aseptically add 10.0mL of sterile vitamin solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Prosthecomicrobium enhy-
drum, Prosthecomicrobium pneumaticum, and Ancalomicrobium species.