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培养基配方
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Medium for Prosthecomicrobium and Ancalomicrobium, Modified
[所属分类:培养基配方] [发布时间:2021-5-20] [发布人:网站管理员2] [阅读次数:] [返回]
Medium for Prosthecomicrobium
and Ancalomicrobium, Modified
Glucose .........................................................................................1.0g
(NH 4 ) 2 SO 4 ...................................................................................0.25g
Peptone........................................................................................0.15g
Yeast extract................................................................................0.15g
Modified Hutner’s basal salts ..................................................20.0mL
Vitamin solution.......................................................................10.0mL
Modified Hutner’s Basal Salts:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O.................................................................................3.34g
FeSO 4 ·7H 2 O..................................................................................0.1g
(NH 4 ) 2 MoO 4 ............................................................................9.25mg
Metals “44”..............................................................................50.0mL
Preparation of Modified Hutner’s Basal Salts: Add nitrilotria-
cetic acid to 500.0mL of distilled/deionized water. Dissolve by adjust-
ing pH to 6.5 with KOH. Add remaining components. Readjust pH to
7.2 with H 2 SO 4 or KOH. Add distilled/deionized water to 1.0L. Store
at 5°C.
Metals “44”:
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.................................................................................1.1g
FeSO 4 ·7H 2 O..................................................................................0.5g
EDTA..........................................................................................0.25g
MnSO 4 ·7H 2 O............................................................................0.154g
CuSO 4 ·5H 2 O...............................................................................0.04g
Co(NO 3 ) 2 ·6H 2 O ........................................................................0.025g
Na 2 B 4 O 7 ·10H 2 O........................................................................0.018g
Preparation of Metals “44”: Add a few drops of H 2 SO 4 to dis-
tilled/deionized water to inhibit precipitate formation. Add compo-
nents to acidified distilled/deionized water and bring volume to
100.0mL. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Thiamine·HCl ............................................................................5.0mg
D- Calcium pantothenate.............................................................5.0mg
Riboflavin ..................................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
room temperature. Aseptically add 10.0mL of sterile vitamin solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Ancalomicrobium ade-
tum, Prosthecomicrobium hirschii, and Prosthecomicrobium species.
and Ancalomicrobium, Modified
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ............................................................................................15.0gGlucose .........................................................................................1.0g
(NH 4 ) 2 SO 4 ...................................................................................0.25g
Peptone........................................................................................0.15g
Yeast extract................................................................................0.15g
Modified Hutner’s basal salts ..................................................20.0mL
Vitamin solution.......................................................................10.0mL
Modified Hutner’s Basal Salts:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................29.7g
Nitrilotriacetic acid.....................................................................10.0g
CaCl 2 ·2H 2 O.................................................................................3.34g
FeSO 4 ·7H 2 O..................................................................................0.1g
(NH 4 ) 2 MoO 4 ............................................................................9.25mg
Metals “44”..............................................................................50.0mL
Preparation of Modified Hutner’s Basal Salts: Add nitrilotria-
cetic acid to 500.0mL of distilled/deionized water. Dissolve by adjust-
ing pH to 6.5 with KOH. Add remaining components. Readjust pH to
7.2 with H 2 SO 4 or KOH. Add distilled/deionized water to 1.0L. Store
at 5°C.
Metals “44”:
Composition per 100.0mL:
ZnSO 4 ·7H 2 O.................................................................................1.1g
FeSO 4 ·7H 2 O..................................................................................0.5g
EDTA..........................................................................................0.25g
MnSO 4 ·7H 2 O............................................................................0.154g
CuSO 4 ·5H 2 O...............................................................................0.04g
Co(NO 3 ) 2 ·6H 2 O ........................................................................0.025g
Na 2 B 4 O 7 ·10H 2 O........................................................................0.018g
Preparation of Metals “44”: Add a few drops of H 2 SO 4 to dis-
tilled/deionized water to inhibit precipitate formation. Add compo-
nents to acidified distilled/deionized water and bring volume to
100.0mL. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Thiamine·HCl ............................................................................5.0mg
D- Calcium pantothenate.............................................................5.0mg
Riboflavin ..................................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
room temperature. Aseptically add 10.0mL of sterile vitamin solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Ancalomicrobium ade-
tum, Prosthecomicrobium hirschii, and Prosthecomicrobium species.