Methanobacterium thermoautotrophicum Medium, Taylor and Pirt

[所属分类:培养基配方] [发布时间:2021-5-24] [发布人:网站管理员2] [阅读次数:] [返回]

Methanobacterium thermoautotrophicum Medium,
Taylor and Pirt

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 CO 3 .........................................................................................4.0g
(NH 4 ) 2 SO 4 .....................................................................................3.0g
NaCl..............................................................................................1.2g
KH 2 PO 4 .........................................................................................0.6g
L -Cysteine·HCl·H 2 O .....................................................................0.5g
K 2 HPO 4 .........................................................................................0.3g
Nitrilotriacetic acid.....................................................................0.03g
CoCl 2 ...........................................................................................0.02g
CaCl 2 ...........................................................................................0.01g
FeSO 4 ..........................................................................................0.01g
MgSO 4 ........................................................................................0.01g
MnSO 4 ........................................................................................0.01g
ZnSO 4 ........................................................................................2.0mg
Resazurin...................................................................................1.0mg
AlK(SO 4 ) 2 ..................................................................................0.2mg
CuSO 4 ........................................................................................0.2mg
H 3 BO 3 ........................................................................................0.2mg
Na 2 MoO 4 ...................................................................................0.2mg
Na 2 S·9H 2 O solution.................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.5g
Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Prepare and distribute medium anaerobi-
cally under 80% H 2 + 20% CO 2 . Add components, except Na 2 S·9H 2 O
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Continue boiling un-
til resazurin turns colorless, indicating reduction. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 10.0mL of
sterile Na 2 S·9H 2 O solution. Mix thoroughly. Aseptically and anaerobi-
cally distribute into sterile tubes or flasks.
Use: For the cultivation of Methanobacterium thermoautotrophicum.