Methanobacterium thermoautotrophicum MS Medium

[所属分类:培养基配方] [发布时间:2021-5-24] [发布人:网站管理员2] [阅读次数:] [返回]

Methanobacterium thermoautotrophicum MS Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaHCO 3 ........................................................................................8.4g
Pancreatic digest of casein............................................................2.0g
Yeast extract..................................................................................2.0g
MgCl 2 ·6H 2 O .................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
K 2 HPO 4 .......................................................................................0.84g
CaCl 2 ·2H 2 O ..................................................................................0.4g
Reazurin....................................................................................0.001g
Trace minerals solution..............................................................1.0mL
pH 7.1 ± 0.2 at 25°C
Trace Minerals Solution:
Composition per liter:
Disodium EDTA·2H 2 O.................................................................0.5g
ZnSO 4 ·2H 2 O...............................................................................0.21g
CoCl 2 ·6H 2 O................................................................................0.15g
AlK(SO 4 ) 2 ...................................................................................0.14g
FeSO 4 ·7H 2 O..................................................................................0.1g
MnSO 4 ·H 2 O..................................................................................0.1g
Na 2 WO 4 ·2H 2 O............................................................................0.03g
CuCl 2 ·2H 2 O................................................................................0.02g
NiCl 2 ·6H 2 O.................................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 2 ·2H 2 O..........................................................................0.01g
Na 2 SeO 4 ......................................................................................0.01g
Preparation of Trace Minerals Solution:  Add components, ex-
cept MnSO 4 ·H 2 O, to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Adjust pH to 7.1. Add MnSO 4 ·H 2 O. Mix thor-
oughly.
Preparation of Medium: Prepare and dispense medium under 80%
H 2 + 20% CO 2 . Add components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Con-
tinue boiling for 3 min. Cool to room temperature while sparging with
80% H 2 + 20% CO 2 . Adjust pH to 7.1 with 6N HCl. Anaerobically dis-
tribute into serum bottles fitted with butyl rubber stoppers and alumi-
num seals. Do not grease stoppers. Autoclave for 15 min at 15 psi
pressure–121°C. Store at room temperature in an anaerobic chamber.
Use:  For the cultivation of Methanobacterium thermoautotrophicum.