Middlebrook 7H10 Agar with Streptomycin

[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]

Middlebrook 7H10 Agar with Streptomycin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Na 2 HPO 4 .......................................................................................1.5g
KH 2 PO 4 .........................................................................................1.5g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
L- Glutamic acid.............................................................................0.5g
Sodium citrate...............................................................................0.4g
Ferric ammonium citrate.............................................................0.04g
MgSO 4 ·7H 2 O............................................................................0.025g
ZnSO 4 ·7H 2 O..............................................................................1.0mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
Pyridoxine..................................................................................1.0mg
Biotin .........................................................................................0.5mg
CaCl 2 ·2H 2 O................................................................................0.5mg
Malachite Green.......................................................................0.25mg
Glycerol .....................................................................................5.0mL
Streptomycin..........................................................................100.0mg
pH 6.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add glycerol to 1.0L of distilled/deion-
ized water and add remaining components. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Aseptically add streptomycin. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation, cultivation, and maintenance of Mycobacte-
rium kansasii.