Middlebrook 7H11 Agar, Selective

[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]

Middlebrook 7H11 Agar, Selective

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Na 2 HPO 4 .......................................................................................1.5g
KH 2 PO 4 .........................................................................................1.5g
Pancreatic digest of casein............................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
L– Glutamic acid ............................................................................0.5g
Sodium citrate...............................................................................0.4g
MgSO 4 ·7H 2 O..............................................................................0.05g
Ferric ammonium citrate.............................................................0.04g
Pyridoxine..................................................................................1.0mg
ZnSO 4 ·7H 2 O..............................................................................1.0mg
CuSO 4 ·5H 2 O..............................................................................1.0mg
CaCl 2 ·2H 2 O................................................................................0.5mg
Malachite Green.......................................................................0.25mg
D– Biotin.......................................................................................0.5μg
Middlebrook OADC enrichment...........................................100.0mL
Antibiotic solution ...................................................................10.0mL
Glycerol .....................................................................................5.0mL
pH 6.6 ± 0.2 at 25°C
Middlebrook OADC Enrichment:
Composition per 100.0mL:
Bovine albumin fraction V...........................................................5.0g
Glucose.........................................................................................2.0g
NaCl............................................................................................0.85g
Oleic acid....................................................................................0.05g
Catalase......................................................................................4.0mg
Source:  This enrichment is available as a prepared enrichment from
BD Diagnostic Systems.
Preparation of Middlebrook OADC Enrichment: Add com-
ponents to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Antibiotic Solution:
Composition per 10.0mL:
Carbenicillin ............................................................................0.05mg
Trimethoprim lactate................................................................0.02mg
Amphotericin B .......................................................................0.01mg
Polymyxin B........................................................................ 200,000U
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add glycerol to 890.0mL of distilled/de-
ionized water and add remaining components, except Middlebrook
OADC enrichment and antibiotic solution. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile Middle-
brook OADC enrichment and 10.0mL of sterile antibiotic solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the selective isolation and cultivation of pathogenic myco-
bacteria from specimens potentially contaminated with bacteria and
fungi.