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Middlebrook 7H11 Agar with Middlebrook OADC Enrichment (Mycobacteria 7H11 Agar with Middlebrook OADC Enrichment)
[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]
Middlebrook 7H11 Agar with
Middlebrook OADC Enrichment
(Mycobacteria 7H11 Agar with
Middlebrook OADC Enrichment)
Na 2 HPO 4 .......................................................................................1.5g
KH 2 PO 4 .........................................................................................1.5g
Pancreatic digest of casein............................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
L -Glutamic acid.............................................................................0.5g
Sodium citrate...............................................................................0.4g
MgSO 4 ·7H 2 O..............................................................................0.05g
Ferric ammonium citrate.............................................................0.04g
Pyridoxine..................................................................................1.0mg
Malachite Green.......................................................................0.25mg
D -Biotin.......................................................................................0.5μg
Middlebrook OADC enrichment...........................................100.0mL
Glycerol .....................................................................................5.0mL
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Middlebrook OADC Enrichment:
Composition per 100.0mL:
Bovine albumin fraction V ...........................................................5.0g
Glucose .........................................................................................2.0g
NaCl............................................................................................0.85g
Oleic acid....................................................................................0.05g
Catalase......................................................................................4.0mg
Source: This enrichment is available as a prepared enrichment from
BD Diagnostic Systems.
Preparation of Middlebrook OADC Enrichment: Add com-
ponents to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add glycerol to 900.0mL of distilled/de-
ionized water and add remaining components, except Middlebrook
OADC enrichment. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 100.0mL of sterile Middlebrook OADC enrichment.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the cultivation of drug-resistant (isoniazid [INH]) strains of
Mycobacterium tuberculosis. For the cultivation of particularly fastid-
ious strains of tubercle bacilli that occur following treatment of tuber-
culosis patients with secondary antitubercular drugs. Generally, these
strains fail to grow on 7H10 medium.
Middlebrook OADC Enrichment
(Mycobacteria 7H11 Agar with
Middlebrook OADC Enrichment)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ............................................................................................15.0gNa 2 HPO 4 .......................................................................................1.5g
KH 2 PO 4 .........................................................................................1.5g
Pancreatic digest of casein............................................................1.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
L -Glutamic acid.............................................................................0.5g
Sodium citrate...............................................................................0.4g
MgSO 4 ·7H 2 O..............................................................................0.05g
Ferric ammonium citrate.............................................................0.04g
Pyridoxine..................................................................................1.0mg
Malachite Green.......................................................................0.25mg
D -Biotin.......................................................................................0.5μg
Middlebrook OADC enrichment...........................................100.0mL
Glycerol .....................................................................................5.0mL
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Middlebrook OADC Enrichment:
Composition per 100.0mL:
Bovine albumin fraction V ...........................................................5.0g
Glucose .........................................................................................2.0g
NaCl............................................................................................0.85g
Oleic acid....................................................................................0.05g
Catalase......................................................................................4.0mg
Source: This enrichment is available as a prepared enrichment from
BD Diagnostic Systems.
Preparation of Middlebrook OADC Enrichment: Add com-
ponents to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add glycerol to 900.0mL of distilled/de-
ionized water and add remaining components, except Middlebrook
OADC enrichment. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 100.0mL of sterile Middlebrook OADC enrichment.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the cultivation of drug-resistant (isoniazid [INH]) strains of
Mycobacterium tuberculosis. For the cultivation of particularly fastid-
ious strains of tubercle bacilli that occur following treatment of tuber-
culosis patients with secondary antitubercular drugs. Generally, these
strains fail to grow on 7H10 medium.
