编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

Middlebrook 7H11 Agar with Middlebrook OADC Enrichment and Triton™ WR 1339 (Mycobacteria 7H11 Agar with Middlebrook OADC Enrichment and Triton™ WR 1339)

[所属分类:培养基配方] [发布时间:2021-5-31] [发布人:网站管理员2] [阅读次数:] [返回]

Middlebrook 7H11 Agar with
Middlebrook OADC Enrichment
and Triton™ WR 1339
(Mycobacteria 7H11 Agar with Middlebrook OADC
Enrichment and Triton™ WR 1339)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Na 2 HPO 4 .......................................................................................1.5g
KH 2 PO 4 .........................................................................................1.5g
Pancreatic digest of casein............................................................1.0g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
L -Glutamic acid ............................................................................0.5g
Sodium citrate...............................................................................0.4g
MgSO 4 ·7H 2 O..............................................................................0.05g
Ferric ammonium citrate.............................................................0.04g
Pyridoxine..................................................................................1.0mg
Malachite Green.......................................................................0.25mg
D -Biotin.......................................................................................0.5μg
Middlebrook OADC enrichment
with Triton™ WR 1339...................................................100.0mL
Glycerol .....................................................................................5.0mL
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Middlebrook OADC Enrichment with
Triton™ WR 1339:
Composition per 100.0mL:
Bovine albumin fraction V ...........................................................5.0g
Glucose.........................................................................................2.0g
NaCl............................................................................................0.85g
Triton™ WR 1339......................................................................0.25g
Oleic acid....................................................................................0.05g
Catalase......................................................................................4.0mg
Source: This enrichment is available as a prepared enrichment from
BD Diagnostic Systems.
Preparation of Middlebrook OADC Enrichment with Tri-
ton™ WR 1339: Add components to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add glycerol to 900.0mL of distilled/de-
ionized water and add remaining components, except Middlebrook
OADC enrichment with Triton™ WR-1339. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile Middle-
brook OADC enrichment with Triton™ WR-1339. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of drug-resistant (isoniazid [INH]) strains of
Mycobacterium tuberculosis. For the cultivation of particularly fastidious
strains of tubercle bacilli that occur following treatment of tuberculosis
patients with secondary antitubercular drugs. Generally, these strains fail
to grow on 7H10 medium.

在线客服

Middlebrook 7H11 Agar with Middlebrook OADC Enrichment and Triton™ WR 1339 (Mycobacteria 7H11 Agar with Middlebrook OADC Enrichment and Triton™ WR 1339)