Mineral Base E for Heterotrophic Growth

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Base E for Heterotrophic Growth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Noble agar...................................................................................15.0g
K 2 HPO 4 .........................................................................................1.2g
KH 2 PO 4 .....................................................................................0.624g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
NaCl..............................................................................................0.1g
CaCl 2 ·6H 2 O solution................................................................10.0mL
MgSO 4 ·7H 2 O solution.............................................................10.0mL
Sodium pyruvate solution........................................................10.0mL
Mineral solution.........................................................................1.0mL
p-Aminobenzoic acid solution...................................................1.0mL
CaCl 2 ·6H 2 O Solution:
Composition per liter:
CaCl 2 ·6H 2 O...................................................................................5.0g
Preparation of CaCl 2 ·6H 2 O  Solution :  Add CaCl 2 ·6H 2 O to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C.
MgSO 4 ·7H 2 O Solution:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................20.0g
Preparation of MgSO 4 ·7H 2 O Solution:  Add MgSO 4 ·7H 2 O to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Sodium Pyruvate Solution:
Composition per 10.0mL:
Sodium pyruvate...........................................................................2.0g
Preparation of Sodium Pyruvate Solution: Add sodium pyru-
vate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
p-Aminobenzoic Acid Solution:
Composition per 10.0mL:
p-Aminobenzoic acid.............................................................100.0mg
Preparation of p-Aminobenzoic Acid Solution: Add p-amin-
obenzoic acid to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C.
Mineral Solution:
Composition per 100.0mL:
Disodium EDTA.........................................................................1.58g
ZnSO4·7H2O................................................................................0.7g
MnSO 4 ·4H 2 O..............................................................................0.18g
FeSO 4 ·7H 2 O................................................................................0.16g
CoCl 2 ·6H 2 O..............................................................................0.052g
Na 2 MoO 4 ·2H 2 O........................................................................0.047g
CuSO 4 ·5H 2 O.............................................................................0.047g
Preparation of Medium:  Add components, except CaCl 2 ·6H 2 O so-
lution, MgSO 4 ·7H 2 O solution, sodium pyruvate solution, and p-amin-
obenzoic acid solution, to distilled/deionized water and bring volume
to 969.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add in the following order:
10.0mL of the sterile CaCl 2 ·6H 2 O solution, 10.0mL of the sterile
MgSO 4 ·7H 2 O solution, 10.0mL of sterile sodium pyruvate solution,
and 1.0mL of sterile p-aminobenzoic acid solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use:  For the heterotrophic cultivation and maintenance of Pseudomo-
nas thermocarboxydovorans.