Mineral Lactate Medium

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Lactate Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

K 2 HPO 4 ·3H 2 O ............................................................................1.13g
NaCl..............................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
KH 2 PO 4 .......................................................................................0.88g
MgSO 4 ·7H 2 O............................................................................... 0.5g
Sodium lactate ..............................................................................0.5g
CaCl 2 ·2H 2 O ...............................................................................5.0mg
Trace elements solution.............................................................1.2mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
Disodium EDTA .........................................................................50.0g
ZnSO 4 ·7H 2 O...............................................................................22.0g
CaCl 2 ·2H 2 O.................................................................................5.54g
MnCl 2 ·4H 2 O................................................................................5.06g
FeSO 4 ·7H 2 O..................................................................................5.0g
CoCl 2 ·6H 2 O ................................................................................1.61g
CuSO 4 ·5H 2 O...............................................................................1.57g
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O....................................................................1.1g
Preparation of Trace Elements Solution : Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 7.0 with KOH.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation and maintenance of Pseudomonas species and
Spirillum species.