Mineral Medium

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract..................................................................................2.0g
Mineral base 5X.....................................................................200.0mL
Trace elements solution SL-6 ....................................................1.0mL
Thiamine·HCl .............................................................................3.0μg
Biotin ..........................................................................................0.2μg
pH 6.8 ± 0.2 at 25°C
Mineral Base 5X:
Composition per liter:
NaCl..............................................................................................5.0g
NH 4 Cl ...........................................................................................2.0g
KH 2 PO 4 .......................................................................................1.35g
MgSO 4 ·7H 2 O................................................................................1.0g
K 2 HPO 4 .......................................................................................0.87g
CaCl 2 ...........................................................................................0.05g
FeCl 3 ·6H 2 O..............................................................................1.25mg
Preparation of Mineral Base 5X:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 · 2H 2 O.................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Arthrobacter species.