Mineral Medium

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NH 4 Cl ...........................................................................................0.5g
Yeast extract..................................................................................0.2g
1,4-Dichlorobenzene.....................................................................0.1g
Na 2 HPO 4 ·7H 2 O......................................................................670.0mg
KH 2 PO 4 ..................................................................................340.0mg
MgSO 4 ·7H 2 O.........................................................................112.0mg
CaCl 2 ........................................................................................14.0mg
ZnSO 4 ·7H 2 O..............................................................................5.0mg
Na 2 MoO 4 ·2H 2 O.........................................................................2.5mg
FeCl 3 ........................................................................................0.13mg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of dichlorobenzene-degrading Pseudomonas
species.