Mineral Medium (DSMZ Medium 994)

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium
(DSMZ Medium 994)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

D -Glucose......................................................................................3.6g
KH 2 PO 4 .........................................................................................1.0g
NH 4 Cl ...........................................................................................0.6g
NaCl..............................................................................................0.4g
MgSO 4 ·7H 2 O................................................................................0.4g
CaCl 2 ·2H 2 O ...............................................................................0.5mg
Vitamin solution.........................................................................5.0mL
Trace elements solution SL-4....................................................1.0mL
pH 6.8 ± 0.2 at 25°C
Vitamin Solution:
Composition per liter:
Thiamine-HCl·2H 2 O................................................................50.0mg
Riboflavin................................................................................50.0mg
Vitamin B 12 ..............................................................................50.0mg
D -Ca-pantothenate ...................................................................50.0mg
p-Aminobenzoic acid...............................................................50.0mg
Lipoic acid...............................................................................50.0mg
Nicotinic acid...........................................................................25.0mg
Niconinamide...........................................................................25.0mg
Biotin.......................................................................................20.0mg
Folic acid .................................................................................20.0mg
Pyridoxine-HCl........................................................................10.0mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly for several
hours. Filter sterilize.
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O................................................................................. 0.2g
Trace elements solution SL-6................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except vitamin and
trace elements solutions, to distilled/deionized water and bring volume
to 994.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to room temperature. Aseptically add sterile trace elements
and vitamin solutions. Mix thoroughly. Adjust pH to 6.8. Aseptically dis-
pense into tubes, flasks, or bottles.
Use:  For the cultivation of Stenotrophomonas maltophilia.