Mineral Medium (DSMZ Medium 1007)

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium
(DSMZ Medium 1007)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

KNO 3 ..........................................................................................0.25g
KH 2 PO 4 .........................................................................................0.1g
MgSO 4 ·7H 2 O..............................................................................0.05g
CaCl 2 ·2H 2 O.................................................................................0.01g
Methanol..................................................................................75.0mL
Trace elements solution .............................................................1.0mL
pH 5.7 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
EDTA............................................................................................5.0g
FeSO 4 ·7H 2 O................................................................................. 2.0g
CoCl 2 ·6H 2 O ..................................................................................0.2g
CuCl 2 ·2H 2 O ..................................................................................0.1g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
H 3 BO 3 .........................................................................................0.03g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
Preparation of Trace Elements Solution:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except trace elements
solution, to distilled/deionized water and bring volume to 999.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
room temperature. Aseptically add sterile trace elements solution. Mix
thoroughly. Adjust pH to 5.7. Aseptically dispense into tubes, flasks, or
bottles. Note: Methane can be substituted for methanol for the growth of
some strains.
Use:  For the cultivation of Methylocella tundrae and Methylocystis hey-
eri.