Mineral Medium with Atrazine (DSMZ Medium 465i)

[所属分类:培养基配方] [发布时间:2021-6-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mineral Medium with Atrazine
(DSMZ Medium 465i)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·2H 2 O.............................................................................3.5g
Na-citrate ......................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
MgCl 2 ·6H 2 O..................................................................................0.1g
CaCl 2 ...........................................................................................0.05g
Atrazine solution......................................................................10.0mL
Trace elements solution SL-4 ....................................................1.0mL
pH 7.25 ± 0.2 at 25°C
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.2g
Trace elements solution SL-6 ................................................100.0mL
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Atrazine Solution:
Composition per 10.0mL:
Atrazine.....................................................................................100mg
Preparation of Atrazine Solution: Add (2-chloro-4(ethylamino)-
6-(isopropylamino)-1,3,5-triazine) to 10.0mL methanol. Mix thor-
oughly. Shortly sonicate to reduce particle size.
Preparation of Medium:  Add components, except atrazine solu-
tion, to 990.0mLL distilled/deionized water. Adjust pH to 7.25. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Aseptically add 10.0mL atazine solution. Mix thoroughly. Aseptically
distribute to sterile tubes or flasks.
Use: For the cultivation of Pseudomonas sp. and other atrazine-utiliz-
ing bacteria.